Generator

Part:BBa_K4761222:Design

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-30)


adh6-linker3-ARO10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2895
    Illegal SpeI site found at 1846
    Illegal PstI site found at 1724
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2895
    Illegal SpeI site found at 1846
    Illegal PstI site found at 1724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2895
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2895
    Illegal SpeI site found at 1846
    Illegal PstI site found at 1724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2895
    Illegal SpeI site found at 1846
    Illegal PstI site found at 1724
    Illegal NgoMIV site found at 991
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Stop codon of adh6 deleted for fusion expression.


Source

BBa_K4761000, BBa_K4761001, BBa_K4761032.

References